PICA Day 23: Neurospora crassa
Cleaned the final soot off the surface of the vessel. The fineness of the mycelia is very lovely. It is behaving so differently from in the petri dish, climbing the walls n all.
PICA Day 9
Subcultured Neurospora onto 4 new PDA plates. Blackening on the outside of the vessel from the bunsen burner wiped off with ethanol and paper. Am very relieved. I was not looking forward to a burnt vessel.
Spent the rest of the day in the gallery preparing for Saturday. Asked Leigh if we could open the studio during the Friday evening opening. Really dont want to step on toes or offend anyone, but it is a super opportunity.
PICA Day 9: Neurospora crassa
Subculturing bread mold onto PDA plates in preparation for subculturing into the exhibition vessel.
PICA Day 8: Neurospora crassa
Prepared potato dextrose agar (PDA) for Neurospora crassa vessel and lab growth trials. Heated agar in vessel to reduce bubbles from pouring. Vessel blackened by bunsen burner!
PICA Day 4
Photos courtesy Megan Schlipalius
Just a quikki tonight as it has been a long day and another tomorrow.
Finished the label holders & handles on the tables. Cocked the first one up of course. put them on upside down! managed to salvage it and the others look supa. successfully ambiguous museum/domestic.
Spent morning getting Daphnia, Hydra & Physarum ready to take to the gallery. subcultured the Physarum into the water agar vessel and fed with oat flakes. Already started moving towards food by the time I got to the gallery. Transferred 5 Daphnia to their vessel and fed. was lovely to sit on the couch later in the afternoon and watch them do their larva lamp thing.
wow trippy - watching the first episode of Star Trek Deep Space Nine and there is a shot of “Dax” getting “impregnated” with her symbiont - everything is luscious red - gloves, coats, sheets - her response to receiving the symbiont is quite erotic.
So back to real life - Hydra dont seem to be thriving. Am wondering if it is the culture media I made - it may be different from the Southern Biological media supplied with the original specimens. Need to find out what is in theirs.
Great to have Oron Catts, Loren Kronemyer & Amy Congdon visit and appreciated their generous comments and support.
Planning to bring Arabidopsis & Drosophila in tomorrow; Candida, C. elegans & Neurospora on Monday.
Skyped with Sue & the twins in the gallery. Technology is amazing. Feels like home now.

Thanks to Megs for her patience with my 5pm energy crash. Bad time for decision making.
neurospora - lab experiments photo by M.Schlipalius

